Application of Tubular Centrifuges in Globulin Separation
Introduction
Globulins are a class of proteins with various important physiological functions and are widely used in fields such as biopharmaceuticals, clinical diagnosis, and biological research. Efficiently and accurately separating globulins from complex biological samples is crucial for obtaining high - purity and high - quality globulin products. As an efficient centrifugal separation device, the tubular centrifuge shows unique advantages in the process of globulin separation and provides reliable technical support for the separation and purification of globulins.
Product Parameters
| Model |
GQ75 |
GQ105 |
GQ125 |
GQ150 |
| Drum Diameter |
75mm |
105mm |
125mm |
150mm |
| Rotating Speed of Drum |
19000rpm |
16300rpm |
15000rpm |
14000rpm |
| Centrifugal force |
15200G |
15620G |
15700G |
15600G |
| Capacity |
600L/H |
1200L/H |
1500L/H |
2500L/H |
| Motor |
1.5kw |
2.2KW |
3.0KW |
3.0KW |
| Weight |
300kg |
500kg |
550kg |
600kg |
Dimension |
760*450*1120mm |
840*500*1600mm |
900*550*1600mm |
950*600*1600mm |
We will provide one year's worth of accessories for free. And professional disassembly tools are also available.
Application
Working Principle of Tubular Centrifuges
A tubular centrifuge is mainly composed of a high - speed rotating drum, a driving device, and feeding and discharging systems. Its working principle is based on the principle of centrifugal sedimentation. When a mixed solution containing globulins enters the high - speed rotating drum (the rotational speed can usually reach 15,000 - 50,000 revolutions per minute or even higher) through the feeding port, under the action of a strong centrifugal force field, different components in the solution are subjected to different magnitudes of centrifugal force due to their density differences. The density of target components such as globulins differs from that of other impurities in the solution (such as cell debris, other proteins, polysaccharides, etc.). Substances with higher density rapidly move towards the drum wall and settle under the action of centrifugal force, while the liquid phase with lower density stays closer to the center of the drum. Through a cleverly designed discharging device, the settled globulins can be separated from other impurities, thus achieving effective separation of globulins.
Advantages of Tubular Centrifuges in Globulin Separation
High Separation Efficiency
The extremely high rotational speed enables the tubular centrifuge to generate a strong centrifugal force field, which can quickly separate substances of different densities. In the case of globulin separation, it can separate globulins from a complex mixed solution in a relatively short time, greatly improving production efficiency. For example, when processing a large amount of plasma to extract immunoglobulins, a tubular centrifuge can complete the work in a few hours that traditional separation methods might take several days to finish.
High - Purity Products
Thanks to its powerful centrifugal separation ability, the tubular centrifuge can effectively remove tiny impurities and other interfering proteins in the mixed solution, significantly increasing the purity of the separated globulins. This is particularly crucial for the production of high - purity globulin preparations in the biopharmaceutical field and can meet strict pharmaceutical quality standards. Research shows that the purity of globulins separated by a tubular centrifuge can reach over 95%, while the purity of products obtained by some traditional separation methods may only be between 70% - 80%.
Gentle Separation Process
During the globulin separation process, the operation of the tubular centrifuge is relatively gentle and does not cause significant damage to the biological activity of globulins. The shear force generated during its high - speed rotation is relatively small, which can maintain the natural structure and function of globulins to the greatest extent. This is crucial for application scenarios where the biological activity of globulins needs to be retained (such as for the preparation of therapeutic antibodies or diagnostic reagents).
Application Examples
Biopharmaceutical Field
In the production of monoclonal antibodies, it is necessary to separate monoclonal antibody globulins from cell culture media. The tubular centrifuge first performs preliminary clarification of the cell culture medium to remove most of the cell debris and impurities, and then further conducts fine separation of the supernatant containing antibody globulins to obtain high - purity monoclonal antibodies. For example, a well - known biopharmaceutical company used a tubular centrifuge to increase the production efficiency of monoclonal antibodies by 30% and simultaneously improve the product purity from 90% to 98%, greatly reducing production costs and improving product quality.
Preparation of Clinical Diagnostic Reagents
When preparing globulin reagents for immunodiagnosis, the tubular centrifuge is used to separate specific immunoglobulins from human or animal serum. For example, in the preparation process of hepatitis B surface antibody detection reagents, the tubular centrifuge efficiently separates hepatitis B surface antibody globulins from human serum. After further purification and processing, these globulins are used to produce high - quality hepatitis B diagnostic reagents, providing an accurate and reliable tool for clinical hepatitis B diagnosis.
Biological Research
When studying the relationship between the structure and function of globulins, it is necessary to obtain high - purity globulin samples with retained biological activity. The tubular centrifuge can separate specific globulins from complex biological tissue extracts to meet research needs. For example, when researching the antibacterial mechanism of a new type of globulin, researchers used a tubular centrifuge to separate the globulin from a bacterial culture medium. Subsequent experiments revealed its unique antibacterial pathway, providing a theoretical basis for the development of new antibacterial drugs.
Precautions in Application
Sample Pretreatment
Before using a tubular centrifuge to separate globulins, the samples must be properly pretreated. For example, biological samples should be filtered to remove large - particle impurities to prevent them from clogging the centrifuge feeding pipeline or affecting the separation effect. In addition, adjusting the pH value and ionic strength of the sample to make it suitable for the stable existence of globulins helps to improve the separation efficiency and product quality.
Equipment Maintenance and Upkeep
Regularly maintain and service the tubular centrifuge to ensure its normal operation. Check the wear of the drum and replace severely worn parts in time to prevent safety accidents caused by drum rupture. At the same time, inspect and maintain key components such as the driving device and seals to ensure the stability and sealing of the equipment and maintain good separation performance.
Optimization of Operating Parameters
According to different globulin samples and separation requirements, precisely optimize the operating parameters of the tubular centrifuge, such as rotational speed, feeding speed, and temperature. An excessively high rotational speed may cause globulin denaturation, while an overly fast feeding speed will affect the separation effect. Determining the optimal operating parameters through experiments can achieve efficient and high - quality separation of globulins.
Conclusion
With its unique working principle and significant advantages, the tubular centrifuge plays a crucial role in the field of globulin separation. From biopharmaceuticals to clinical diagnosis and biological research, the tubular centrifuge provides a powerful technical means for obtaining high - purity and high - activity globulins, promoting the rapid development of related fields. With the continuous progress of technology, the performance and intelligent control of tubular centrifuges will be continuously optimized. It is expected to show broader application prospects in globulin separation and other biological separation fields and make greater contributions to human health and biological science research.
Pharmaceutical industry
Vaccine production: In the process of vaccine preparation, microbial cells, impurities, etc. need to be separated from the culture medium. GQ tubular centrifuge can effectively achieve solid-liquid separation, obtain high-purity vaccine products, and ensure the quality and safety of vaccines, such as the production of influenza vaccine, hepatitis B vaccine, etc
Antibiotic extraction: Used for separating and extracting antibiotics from fermentation broth, removing solid impurities such as mycelium and culture medium residue, improving the purity and yield of antibiotics, commonly used in the production process of antibiotics such as penicillin and cephalosporins.
Traditional Chinese Medicine Preparations: Traditional Chinese medicine extracts often contain impurities that are difficult to separate, such as soap, protein, polysaccharides, etc. GQ tube centrifuge can quickly and effectively remove these impurities without losing effective ingredients, improve the clarity and quality of the medicine, shorten the settling time, and increase production. It is widely used in the production of various oral and injection formulations of traditional Chinese medicine
Biological products industry
Bacterial Separation: In microbial fermentation production, it can be used to separate and collect bacterial cells, such as yeast and lactic acid bacteria, for subsequent bacterial processing or extraction of metabolites. It has the characteristics of good separation effect and high recovery rate, which helps to improve production efficiency and product quality
Enzyme extraction: The production of highly active and high-purity enzyme preparations, such as amylase and protease, by separating enzyme proteins from fermentation broth or biological tissue homogenates containing enzymes, removing impurities and cell debris
Blood product processing: Used for centrifugal separation of plasma cold precipitation, it can effectively separate useful components such as coagulation factors in plasma. For example, GQ142 high-speed tubular separator can meet the requirements of cold precipitation extraction process in plasma cold precipitation centrifugal process, ensuring the quality and yield of cold precipitation
Food and beverage industry
Juice clarification: It can quickly remove impurities such as fruit pulp fibers and pectin from juice, making the juice clearer and more transparent, improving the quality and stability of juice, and extending the shelf life of juice. It is suitable for the production of various juices such as apple juice, orange juice, grape juice, etc
Refining edible oil: It can be used to remove impurities such as phospholipids, proteins, and colloids from edible oil, improve the transparency and quality of edible oil, and play an important role in the refining process of edible oils such as soybean oil, rapeseed oil, and peanut oil
Beverage production: In the production of tea beverages, coffee beverages, etc., it is used to separate and extract the active ingredients, remove residues and impurities, and make the beverage taste more pure and the quality more stable.
Chemical industry
Production of pigments and dyes: In the synthesis process of pigments and dyes, it is necessary to separate the solid particles after reaction from the liquid solvent. GQ tube centrifuge can achieve efficient solid-liquid separation, improve the purity and quality of pigments and dyes, and ensure the color and performance of the product
Catalyst recovery: used to separate and recover catalysts from mixtures after chemical reactions for recycling, reducing production costs, and improving production efficiency. It has a wide range of applications in petrochemical, fine chemical, and other fields.
Polymer material synthesis: In the synthesis process of polymer materials, it can be used to remove unreacted monomers, initiators and other impurities, as well as separate polymers of different molecular weights, to improve the performance and quality of products.
Detailed Photos
FAQ
Q1.What's your advantage? Why we choose you?
A:1) More than 50 years experience of centrifuge & separator production and R&D.We have the most professional techniques and team. More advantage in price.
2)Great production capacity Sustainable Supply . According to customer requirements, We support the customized include package.
3)We use Internationally renowned brand Inverter and PLC . Good quality materials and the best production process
Q2.What's the Payment?
A: T/T, Western Union, L/C, Ali Pay, etc
Q3.Are you a factory or trading company?
A:We are a professional manufacturer. We are warmly welcome clients from worldwide to visit our factory and cooperate with us.
Q4.How long is the warranty period ?
A:18 months after install .
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