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Steps and Applications of Separating Bacteria Using a Tubular Centrifuge
A tubular centrifuge can be used to separate bacterial cells from liquid components in bacterial cultures, making it suitable for fields such as biopharmaceuticals, food industry, and environmental monitoring. Below are the detailed steps and precautions:
Pre-Treatment:
Culture Preparation: Perform preliminary filtration of the bacterial liquid (e.g., fermentation broth) to remove large particulate impurities.
Condition Adjustment: Adjust the pH or temperature of the culture based on the bacterial type to ensure cell viability (if live bacteria need to be preserved).
Centrifugal Separation:
Feeding: Inject the pre-treated bacterial liquid into the feeding system of the tubular centrifuge.
Parameter Setting: Adjust the rotational speed to 15,000-30,000 rpm (depending on the bacterial species and centrifuge model) to precipitate the bacteria using centrifugal force.
Layering Results:
Bacterial Pellet: Deposited on the inner wall of the centrifuge tube.
Supernatant: Discharged through the outlet, containing soluble metabolites or impurities.
Collection and Post-Treatment:
Bacterial Pellet Collection: Scrape the pellet using sterile tools and resuspend it in a buffer or culture medium.
Supernatant Treatment: If the supernatant contains target products (e.g., extracellular enzymes), further purification can be performed; otherwise, dispose of it according to biosafety standards.
Equipment Cleaning: Thoroughly sterilize the centrifuge to avoid cross-contamination.
High-Efficiency Concentration: Rapid separation of large volumes of bacterial cells, suitable for industrial production.
High Purity: Removes impurities from the culture, improving bacterial purity.
Flexibility: Adaptable to different bacterial species by optimizing rotational speed and time.
Continuous Operation: Some models support continuous feeding, enhancing processing efficiency.
Parameter Optimization: Different bacteria (e.g., E. coli vs. Lactobacillus) vary in density and size, requiring pre-experiments to determine the optimal speed and time.
Temperature Control: High-speed centrifugation may generate heat; enable cooling (4-10°C) to protect heat-sensitive bacteria.
Aseptic Operation: Perform all steps in a biosafety cabinet or clean environment to avoid contamination.
Biosafety: If separating pathogenic bacteria, adhere to Biosafety Level (BSL) guidelines and inactivate waste liquids/residues.
Biopharmaceuticals: Collect engineered bacteria (e.g., E. coli expressing recombinant proteins) for downstream purification.
Food Industry: Separate probiotics (e.g., Lactobacillus) for yogurt or probiotic supplement production.
Environmental Monitoring: Concentrate bacteria in water samples for pathogen detection or microbial analysis.
Research Experiments: Extract bacterial cells for genomic or proteomic studies.
High-Speed Benchtop Centrifuge: Suitable for small-volume, lab-scale separation but limited in processing capacity.
Continuous Flow Centrifuge: More suitable for large-scale continuous production but higher equipment costs.
Ultracentrifuge: Used for nanoscale particles (e.g., viruses) but typically unnecessary for bacterial separation.
By using a tubular centrifuge appropriately, efficient bacterial separation can be achieved, balancing purity, viability, and cost. It is a preferred solution for industrial microbial processing.
|
Model
|
Bowl Speed
(rpm) |
Drum Volume
(L) |
Motor
(KW |
Dimensions, Overall (mm)
|
|
75
|
19000
|
2.2
|
1.5
|
760*450*1120
|
|
105
|
16300
|
6
|
2.2
|
840*500*1600
|
|
125
|
15000
|
8
|
3.0
|
900*550*1600
|
|
150
|
14000
|
10
|
3.0
|
1020*600*1600
|
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